{"id":605,"date":"2020-09-21T20:46:24","date_gmt":"2020-09-21T11:46:24","guid":{"rendered":"http:\/\/snsclub.urayasucitizens.net\/mousebuddies\/?p=605"},"modified":"2020-09-21T20:50:17","modified_gmt":"2020-09-21T11:50:17","slug":"%e3%81%82%e3%82%89%e3%81%9f%e3%82%81%e3%81%a6%e5%b8%b8%e8%ad%98%e3%81%ab%e7%95%b0%e8%a6%8b%e3%82%92","status":"publish","type":"post","link":"https:\/\/snsclub.urayasucitizens.net\/mousebuddies\/2020\/09\/21\/%e3%81%82%e3%82%89%e3%81%9f%e3%82%81%e3%81%a6%e5%b8%b8%e8%ad%98%e3%81%ab%e7%95%b0%e8%a6%8b%e3%82%92\/","title":{"rendered":"\u3042\u3089\u305f\u3081\u3066\u5e38\u8b58\u306b\u7570\u898b\u3092"},"content":{"rendered":"

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    \n
  1. \u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u3057\u3066\u3044\u308b\u304b\u3069\u3046\u304b\u306f\u3001\u672c\u6765\u3001\u6297\u539f\u304c\u3042\u308b\u304b\u3069\u3046\u304b\u3092\u6297\u4f53\u3092\u7528\u3044\u3066\u884c\u3046\u3082\u306e\u3060\u304c\u3001<\/span><\/li>\n
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  3. \u305d\u306e\u305f\u3081\u306bPCR\u5206\u6790\u3068\u3044\u3046\u624b\u6cd5\u304c\u5e83\u304f\u4f7f\u308f\u308c\u3066\u3044\u308b\u304c\u3001PCR\u691c\u67fb\u306f\u3042\u304f\u307e\u3067\u3001\u907a\u4f1d\u5b50\u306e\u4e00\u90e8\u5206\u3092\u5897\u5e45\u3055\u305b\u308b\u305f\u3081\u306e\u6280\u8853\u3067\u3042\u308b\u3053\u3068\u3092\u898b\u9003\u3057\u3066\u306f\u306a\u3089\u306a\u3044\u3002\u907a\u4f1d\u5b50\u5224\u5b9a\u6280\u8853\u3092\u3001PCR\u306e\u5c02\u9580\u5bb6\u306f\uff19\uff19\uff05\u306e\u7279\u7570\u5ea6\uff08\uff11\uff05\u306e\u64ec\u967d\u6027\uff09\u3092\u6301\u3064\u6545\u306b\u8a3a\u65ad\u3068\u3057\u3066\u4f7f\u3048\u308b\u3053\u3068\u3092\u5f37\u8abf\u3059\u308b\u304c\u3001\u679c\u305f\u3057\u3066\u305d\u3046\u65ad\u8a00\u3067\u304d\u308b\u3082\u306e\u3060\u308d\u3046\u304b\u3002<\/span><\/li>\n
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  5. \u305d\u306e\u7406\u7531\u3068\u3057\u3066\u5927\u304d\u306a\u554f\u984c\u306f\u3001\u5897\u5e45\u30b5\u30a4\u30af\u30eb\u3092\u4e0a\u3052\u3055\u3048\u3059\u308c\u3070\u3001\u5c11\u306a\u3044\u30a6\u30a4\u30eb\u30b9\u91cf\u3067\u3082\uff08\u5178\u578b\u7684\u306b\u306f\u7121\u75c7\u72b6\u60a3\u8005\u3067\u5371\u967a\u6027\u306f\u7121\u3044\u3082\u306e\u307e\u3067\u3082\uff09\u3001\u967d\u6027\u53cd\u5fdc\u3092\u793a\u3059\u5371\u967a\u6027\u304c\u3042\u308b\u3002<\/span><\/li>\n
  6. \u3053\u306e\u305f\u3081\u3001\u5897\u5e45\u30b5\u30a4\u30af\u30eb\u6570\u3092\u4f4e\u304f\u6291\u3048\u308b\u3001\u4f8b\u3048\u307040\u304b\u308920\u30b5\u30a4\u30af\u30eb\u7a0b\u5ea6\u306b\u3059\u3079\u304d\u3001\u3068\u3044\u3046\u3053\u3068\u3092\u5531\u9053\u3059\u308b\u8b70\u8ad6\u3082\u6210\u308a\u7acb\u3064\u3002<\/span><\/li>\n
  7. \u3053\u3046\u3044\u3063\u305fPCR\u3067\u967d\u6027\u5224\u65ad\uff1d\u611f\u67d3\u5224\u65ad\u3068\u3044\u3046\u77ed\u7d61\u7684\u601d\u8003\u56de\u8def\u3067\u3001\u533b\u5e2b\u7b49\u306e\u4ecb\u5728\u306a\u3057\u306b\u6a5f\u68b0\u7684\u306b\u611f\u67d3\u8005\u3068\u3057\u3066\u5165\u9662\u3055\u305b\u3001\u30de\u30b9\u30af\u30013\u5bc6\u56de\u907f\u3001\u30bd\u30fc\u30b7\u30e3\u30eb\u30c7\u30a3\u30b9\u30bf\u30f3\u30b9\u3068\u3044\u3063\u305f\u30de\u30a4\u30ca\u30b9\u601d\u8003\u3060\u3051\u3092\u690d\u3048\u4ed8\u3051\u308b\u306e\u3067\u306f\u80fd\u304c\u7121\u3044\u3002\u539a\u52b4\u7701\u306f\u3058\u3081\u3001\u653f\u5e9c\u306f\u3053\u3093\u306a\u3053\u3068\u306b\u306f\u627f\u77e5\u306e\u4e0a\u3067\u3001PCR\u3092\u56fd\u6c11\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306e\u4fbf\u5b9c\u7684\u6d3b\u7528\u30c4\u30fc\u30eb\u3068\u3057\u3066\u5229\u7528\u3057\u3066\u3044\u308b\u5074\u9762\u3082\u539a\u52b4\u7701\u767a\u8a00\u306b\u898b\u3089\u308c\u308b\u304c\u3001\u305d\u306e\u4ed8\u3051\u306f\u5fc5\u305a\u5e02\u5834\u306e\u6df7\u4e71\u3068\u9006\u8972\u3068\u306a\u3063\u3066\u8df3\u306d\u8fd4\u308b\u3002<\/span><\/li>\n
  8. \u307e\u305f\u3001\u672c\u8cea\u7684\u306a\u3053\u3068\u306e\u4e00\u3064\u3068\u3057\u3066\u3001\u30a6\u30a4\u30eb\u30b9\u306e\u5909\u7570\u3068\u8a00\u3046\u3053\u3068\u3082\u6307\u6458\u3055\u308c\u3066\u304a\u308a\uff08\u6ce8\uff11<\/span>\uff09\u3001\u30a2\u30df\u30ce\u9178\u3092\u5909\u3048\u306a\u3044\u3088\u3046\u306a\u907a\u4f1d\u5b50\u5909\u7570\uff08\u30a2\u30df\u30ce\u9178\u306e\u540c\u7fa9\u7684\u7f6e\u63db\uff09\u306e\u5834\u5408\u3067\u3082\u3001\u907a\u4f1d\u5b50\u69cb\u9020\u304c\u9055\u3046\u305f\u3081PCR\u3067\u691c\u51fa\u3055\u308c\u306a\u3044\u30ea\u30b9\u30af\u304c\u751f\u3058\u308b\u3002\u3053\u3046\u306a\u308b\u3068\u3001\u7279\u7570\u602799\uff05\u306e\u524d\u63d0\u304c\u5927\u304d\u304f\u58ca\u308c\u3001\u4f55\u3092\u898b\u3066\u3044\u308b\u306e\u304b\u5168\u304f\u5206\u304b\u3089\u306a\u304f\u306a\u308b\u3002<\/span><\/li>\n
  9. \u3053\u306e\u307b\u304b\u3001\u3053\u308c\u306b\u691c\u67fb\u7cbe\u5ea6\u306e\u3082\u3046\u4e00\u3064\u306e\u6307\u6a19\u3067\u3042\u308b\u611f\u5ea6\u304c\u554f\u984c\u3068\u306a\u308a\uff08\u901a\u5e38\u611f\u5ea6\u306f\uff17\uff10\uff05\u3068\u3044\u308f\u308c\u308b\u304c\uff09\u3001\u3053\u308c\u3082\u691c\u67fb\u8a3a\u65ad\u306e\u9053\u5177\u3068\u3057\u3066\u306ePCR\u691c\u67fb\u3092\u63fa\u308b\u304c\u3059\u3053\u3068\u3068\u306a\u308b\u3002<\/span><\/li>\n<\/ol>\n\n\n\n\n
    \n

    \u7279\u7570\u5ea6<\/span><\/h1>\n<\/td>\n

    		\n

    \u7279\u7570\u5ea6\u304c\u9ad8\u3044\u3001\u3068\u306f\u3001\u300c\u9670\u6027\u306e\u3082\u306e\u3092\u6b63\u3057\u304f\u9670\u6027\u3068\u5224\u5b9a\u3059\u308b\u53ef<\/span>\u80fd\u6027\u304c\u9ad8\u3044\u300d\u3001\u3042\u308b\u3044\u306f\u300c\u9670\u6027\u306e\u3082\u306e\u3092\u9593\u9055\u3063\u3066\u967d\u6027\u3068\u5224\u5b9a\u3059\u308b\u53ef\u80fd\u6027\u304c\u4f4e\u3044\u300d\u3068\u3044\u3046\u610f\u5473\u3067\u3042\u308b\u3002<\/span><\/h1>\n<\/td>\n<\/tr>\n

    \u611f\u5ea6<\/b><\/span><\/td>\n\u611f\u5ea6<\/b>\u3068\u306f\u3001\u81e8\u5e8a\u691c\u67fb<\/a>\u306e\u6027\u683c\u3092\u6c7a\u3081\u308b\u6307\u6a19\u306e1\u3064\u3067\u3001\u3042\u308b\u691c\u67fb\u306b\u3064\u3044\u3066\u300c\u967d\u6027\u3068\u5224\u5b9a\u3055\u308c\u308b\u3079\u304d\u3082\u306e\u3092\u6b63\u3057\u304f\u967d\u6027\u3068\u5224\u5b9a\u3059\u308b\u78ba\u7387\u300d\u3068\u3057\u3066\u5b9a\u7fa9\u3055\u308c\u308b\u5024\u3067\u3042\u308b\u3002<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    \uff08\u6ce8\uff11<\/span>\uff09<\/span><\/p>\n

    \u907a\u4f1d\u5b50\u5909\u7570\u306e\u5b9f\u614b\u3000<\/span>https:\/\/royalsocietypublishing.org\/doi\/10.1098\/rsos.200636<\/a><\/span>Published:<\/span>10 June 2020<\/span><\/span>https:\/\/doi.org\/10.1098\/rsos.200636<\/a><\/span><\/p>\n

    Presence of mismatches between diagnostic PCR assays and coronavirus SARS-CoV-2 genome<\/span><\/h1>\n

    \u91cd\u75c7\u6025\u6027\u547c\u5438\u5668\u75c7\u5019\u7fa4\u30b3\u30ed\u30ca\u30a6\u30a4\u30eb\u30b92\uff08SARS-CoV-2;\u6700\u521d\u306f2019-nCoV\u3068\u540d\u4ed8\u3051\u3089\u308c\u305f\uff09\u306f\u3001\u6700\u8fd1\u306eCOVID-19\u30d1\u30f3\u30c7\u30df\u30c3\u30af\u306e\u539f\u56e0\u3067\u3042\u308a\u3001\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u9023\u9396\u53cd\u5fdc\uff08PCR\uff09\u306f\u3001\u60a3\u8005\u306e\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u8a3a\u65ad\u3059\u308b\u305f\u3081\u306e\u73fe\u5728\u306e\u6a19\u6e96\u7684\u306a\u65b9\u6cd5\u3067\u3059\u3002<\/span><\/p>\n

    \u3053\u306e\u7814\u7a76\u3067\u306f\u3001\u516c\u958b\u3055\u308c\u3066\u3044\u308b\u30a6\u30a4\u30eb\u30b9\u914d\u5217\u3068\u306e\u30df\u30b9\u30de\u30c3\u30c1\u306e\u8a55\u4fa1\u3092\u901a\u3058\u3066\u3001\u516c\u958b\u3055\u308c\u3066\u3044\u308bPCR\u8a3a\u65ad\uff08\u4e16\u754c\u4fdd\u5065\u6a5f\u95a2\uff08WHO\uff09\u304c\u63a8\u5968\u3059\u308b\u3082\u306e\u3092\u542b\u307f\uff09\u306e\u518d\u8a55\u4fa1\u3092\u884c\u3044\u307e\u3057\u305f\u3002\u4e16\u754c\u4e2d\u306e17000\u4ee5\u4e0a\u306e\u30a6\u30a4\u30eb\u30b9\u914d\u5217\u3092\u4f7f\u7528\u3057\u3066\u3001\u30a6\u30a4\u30eb\u30b9\u30b2\u30ce\u30e0\u306e\u30d7\u30e9\u30a4\u30de\u30fc\/\u30d7\u30ed\u30fc\u30d6\u30bf\u30fc\u30b2\u30c3\u30c8\u9818\u57df\u5185\u306e\u914d\u5217\u306e\u591a\u69d8\u6027\u3092\u5fb9\u5e95\u7684\u306b\u8a55\u4fa1\u3057\u307e\u3057\u305f\u3002\u5206\u6790\u306b\u3088\u3063\u3066\u300127\u306e\u5206\u6790\u7269\u306e\u3046\u30617\u3064\u306e\u5206\u6790\u7269\u306e\u30d7\u30e9\u30a4\u30de\u30fc\/\u30d7\u30ed\u30fc\u30d6\u7d50\u5408\u9818\u57df\u306b\u5909\u7570\/\u30df\u30b9\u30de\u30c3\u30c1\u306e\u5b58\u5728\u304c\u793a\u3055\u308c\u307e\u3057\u305f<\/strong>\u3002<\/span><\/p>\n

    \n

    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; initially named as 2019-nCoV) is responsible for the recent COVID-19 pandemic and polymerase chain reaction (PCR) is the current standard method for its diagnosis from patient samples. This study conducted a reassessment of published diagnostic PCR assays, including those recommended by the World Health Organization (WHO), through the evaluation of mismatches with publicly available viral sequences. An exhaustive evaluation of the sequence variability within the primer\/probe target regions of the viral genome was performed using more than 17 000 viral sequences from around the world. The analysis showed the presence of mutations\/mismatches in primer\/probe binding regions of 7 assays out of 27 assays studied.<\/strong>\u00a0<\/span><\/p>\n<\/div>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    \u3053\u306e\u30b3\u30ed\u30ca\u9a12\u52d5\u306b\u5bfe\u3059\u308b\u653f\u5e9c\u306e\u59ff\u52e2\u3082\u5f90\u3005\u306b\u5909\u308f\u308a\u3064\u3064\u3042\u308b\u3088\u3046\u306b\u601d\u3046\u3002\u8868\u5411\u304d\u306f\u307e\u3060\u59ff\u52e2\u3092\u5909\u3048\u3066\u3044\u306a\u3044\u304c\u3001\u30d7\u30ed\u91ce\u7403\u3084\u76f8\u64b2\u3001\u5c4b\u5916\u30a4\u30d9\u30f3\u30c8\u306b\u653e\u4efb\u59ff\u52e2\u304c\u8868\u308c\u3066\u304d\u3066\u3044\u3066\u826f\u3044\u3053\u3068\u3060\u3002\u305f\u3060\u3001PCR\u967d\u6027\u3092\u610f\u56f3\u7684\u306b\u611f\u67d3\u3068\u8a00\u3044\u63db\u3048\u3001\u60c5\u5831\u3092\u5782\u308c\u6d41…<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"categories":[8,4,7,5],"tags":[],"class_list":["post-605","post","type-post","status-publish","format-standard","hentry","category-8","category-4","category-7","category-5"],"aioseo_notices":[],"gutentor_comment":0,"_links":{"self":[{"href":"https:\/\/snsclub.urayasucitizens.net\/mousebuddies\/wp-json\/wp\/v2\/posts\/605","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/snsclub.urayasucitizens.net\/mousebuddies\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/snsclub.urayasucitizens.net\/mousebuddies\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/snsclub.urayasucitizens.net\/mousebuddies\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/snsclub.urayasucitizens.net\/mousebuddies\/wp-json\/wp\/v2\/comments?post=605"}],"version-history":[{"count":6,"href":"https:\/\/snsclub.urayasucitizens.net\/mousebuddies\/wp-json\/wp\/v2\/posts\/605\/revisions"}],"predecessor-version":[{"id":613,"href":"https:\/\/snsclub.urayasucitizens.net\/mousebuddies\/wp-json\/wp\/v2\/posts\/605\/revisions\/613"}],"wp:attachment":[{"href":"https:\/\/snsclub.urayasucitizens.net\/mousebuddies\/wp-json\/wp\/v2\/media?parent=605"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/snsclub.urayasucitizens.net\/mousebuddies\/wp-json\/wp\/v2\/categories?post=605"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/snsclub.urayasucitizens.net\/mousebuddies\/wp-json\/wp\/v2\/tags?post=605"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}